bioForNEET • NCERT Prep CLASS XI • CHAPTER 8

CELL — THE UNIT OF LIFE

I. CELL — OVERVIEW & DISCOVERY

A    WHAT IS A CELL?

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FEATURE DETAIL
Definition Fundamental structural & functional unit of all living organisms
Unicellular organisms Composed of a single cell; capable of independent existence & performing all essential functions of life
Multicellular organisms Composed of many cells; exhibit division of labour
Key principle Anything less than a complete cell structure does NOT ensure independent living

B    SCIENTISTS & DISCOVERIES — MASTER TABLE

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SCIENTIST DISCOVERY / CONTRIBUTION YEAR
Robert Hooke Discovered the first cell (dead cork cells) 1665
Antonie van Leeuwenhoek First saw & described a live cell
Robert Brown Discovered the Nucleus 1831
Flemming Gave the name 'Chromatin'
Matthias Schleiden German Botanist; all plants composed of cells 1838
Theodore Schwann German Zoologist; proposed plasma membrane; cell wall unique to plant cells 1839
Schleiden + Schwann Formulated the Cell Theory 1838–39
Rudolf Virchow 'Omnis cellula-e cellula' — cells arise from pre-existing cells ⭐⭐ 1855
Singer & Nicolson Fluid Mosaic Model of plasma membrane 1972
Camillo Golgi Discovered Golgi bodies 1898
George Palade Discovered Ribosomes (dense particles under EM) 1953
G.N. Ramachandran Triple helical structure of collagen; Ramachandran plot 1954
⚡ EXAM TRAP

NEET 2019, RE-NEET 2026 (reinforced): Cell theory was formulated by Schleiden and Schwann (1838-39). 'Omnis cellula-e cellula' = Rudolf Virchow (1855) — all cells arise from pre-existing cells.

⚡ EXAM TRAP

NEET TRAP: Robert Hooke discovered the cell (dead); Robert Brown discovered the nucleus — don't confuse! Schwann is described as a German Zoologist in NCERT.

⚡ EXAM TRAP

NEET 2025: Reductionist Biology is the physico-chemical approach to study living organisms.

C    CELL THEORY

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POINT DETAIL
Formulated bySchleiden & Schwann
Modified byRudolf Virchow (1855)
Statement 1All living organisms are composed of cells and products of cells
Statement 2All cells arise from pre-existing cells

D    CELL SIZES — IMPORTANT NUMBERS

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ORGANISM / CELL SIZE
Mycoplasma (smallest cell)0.3 µm
PPLO (Mycoplasma)~0.1 µm
Typical Bacteria1–2 µm (range: 3–5 µm)
Viruses0.02–0.2 µm
Human RBCs~7.0 µm diameter
Typical Eukaryotic cell10–20 µm
Largest cellOstrich egg
Longest cellNerve cell

II. PROKARYOTIC VS EUKARYOTIC CELLS

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FEATURE PROKARYOTIC EUKARYOTIC
Nuclear membrane Absent Present
Nucleus No well-defined nucleus; genetic material = naked DNA Organised nucleus with nuclear envelope
Membrane-bound organelles Absent Present (ER, Golgi, mitochondria, etc.)
Ribosomes 70S (50S + 30S) 80S (60S + 40S)
Cell wall Present (except Mycoplasma) Present in plants & fungi; absent in animals
Chromosome Single, circular DNA; no histones Linear chromosomes; histones present
Plasmids Present (small circular DNA) Absent
Examples Bacteria, Cyanobacteria, Mycoplasma, PPLO Protists, Plants, Animals, Fungi
⚡ EXAM TRAP

NEET 2015, 2012, RE-NEET 2026 (reinforced): Prokaryotic cells lack nuclear membrane & membrane-bound organelles; have 70S ribosomes (50S + 30S subunits); can have plasmids and mesosomes, but peroxisomes are absent.

⚡ EXAM TRAP

NEET 2026: Ribosomes are non-membrane bound organelles found in both prokaryotic and eukaryotic cells!

ORGANELLE CLASSIFICATION BY MEMBRANE ⭐⭐⭐

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CATEGORY EXAMPLES
Single membrane-bound Vacuole, Lysosome, Golgi apparatus, Endoplasmic Reticulum
Double membrane-bound Nucleus, Mitochondria, Chloroplast
Non-membrane-bound Ribosomes, Nucleolus, Chromatin, Cilia & Flagella, Centrosome
⚡ EXAM TRAP

NEET 2024, 2015: Double membrane-bound = Nucleus, Mitochondria, Chloroplast. Non-membrane-bound = Ribosomes, Centrosome, Nucleolus.

III. PROKARYOTIC CELL — DETAILED

A    CELL ENVELOPE & MODIFICATIONS

Cell envelope = three-layered structure (outside → inside):

🔑 SEQUENCE: GLYCOCALYX (outermost) → CELL WALL → PLASMA MEMBRANE (innermost)
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LAYER DETAIL
GlycocalyxOutermost; differs in composition & thickness

Glycocalyx — Two Forms ⭐

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FORM FEATURE
Slime layerLoose sheath
CapsuleThick & tough

Gram Staining ⭐

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CATEGORY STAINING RESPONSE
Gram positive (+ve)Take up Gram stain
Gram negative (–ve)Do NOT take up Gram stain
BasisDifferences in cell envelope composition
⚡ EXAM TRAP

NEET 2017: Glycocalyx = outermost layer providing sticky character to bacterial cell.

B    CELL WALL — BACTERIA

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FEATURE DETAIL
Composition Peptidoglycan (in bacteria)
Functions Determines shape of cell; provides strong structural support; prevents bursting or collapsing
Exception Mycoplasma — wall-less (lacks cell wall)
⚡ EXAM TRAP

NEET 2016, 2022: Mycoplasma / PPLO — lack cell wall; can pass through filters < 1 µm. Bacterial cell wall = Peptidoglycan.

C    PLASMA MEMBRANE (Prokaryotic)

• Selectively permeable
• Structurally similar to eukaryotic membrane
• Interacts with outside world

D    MESOSOMES

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FEATURE DETAIL
What are they? Extensions of plasma membrane into the cell
Characteristic ofProkaryotes
Three forms Vesicles, Tubules, Lamellae

Functions of Mesosomes ⭐⭐

Cell wall formation
DNA replication & distribution to daughter cells
Respiration
• Secretion processes
Increase surface area of plasma membrane
• Increase enzymatic content

⚡ EXAM TRAP

NEET 2014, 2023, 2024, 2025, RE-NEET 2024: Mesosome is a specialised membranous structure in prokaryotic cell (vesicles, tubules, lamellae) helping in cell wall formation, DNA replication, respiration, and secretion.

⚡ EXAM TRAP

NEET 2026: Extensions of plasma membrane into the cell forming mesosomes occur in prokaryotes — NOT in eukaryotic cell membranes!

Chromatophores ⭐

In some prokaryotes like cyanobacteria → membranous extensions called chromatophores containing pigments.

E    FLAGELLA (Prokaryotic)

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FEATURE DETAIL
StructureThin filamentous extensions from cell wall
Three parts Filament (longest, extends outside), Hook, Basal body
FunctionMotility
NoteStructurally different from eukaryotic flagella

F    PILI & FIMBRIAE

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STRUCTURE FEATURES FUNCTION
Pili Elongated, tubular; made of special protein Attachment (NOT motility)
Fimbriae Small, bristle-like fibres Help bacteria attach to rocks in streams and to host tissues
⚡ EXAM TRAP

NEET 2015, 2016: Pili & Fimbriae — surface structures of bacteria; do NOT play role in motility; help in attachment.

G    RIBOSOMES & INCLUSION BODIES

Ribosomes (Prokaryotic) ⭐

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FEATURE DETAIL
Size~15 nm × 20 nm
Type70S
Subunits50S + 30S
Function Protein synthesis (site of protein synthesis)
LocationAssociated with plasma membrane
Polyribosomes / Polysomes Several ribosomes attached to a single mRNA forming a chain → translate mRNA into proteins
⚡ EXAM TRAP

NEET 2016, 2018: Polysome = multiple ribosomes on single mRNA = multiple copies of polypeptide.

Inclusion Bodies ⭐⭐

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FEATURE DETAIL
NatureReserve material in prokaryotic cells
MembraneNOT bound by any membrane
LocationLie free in cytoplasm
ExamplesPhosphate granules, Cyanophycean granules, Glycogen granules
⚡ EXAM TRAP

NEET 2015, 2020: Inclusion bodies — reserve material; not membrane-bound; lie free in cytoplasm; NOT involved in food ingestion.

Gas Vacuoles ⭐

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FEATURE DETAIL
Found in Blue-green, Purple & Green photosynthetic bacteria

IV. EUKARYOTIC CELL — DETAILED

KEY DIFFERENCES — PLANT CELL vs ANIMAL CELL ⭐⭐

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FEATURE PLANT CELL ANIMAL CELL
Cell wallPresent (cellulose)Absent
PlastidsPresentAbsent
Large central vacuolePresentAbsent or small
CentriolesAbsent in almost all plant cells Present
PlasmodesmataPresentAbsent

A    CELL MEMBRANE (Plasma Membrane)

Fluid Mosaic Model (Singer & Nicolson, 1972) ⭐⭐⭐

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FEATURE DETAIL
Model proposed bySinger & Nicolson (1972)
Main compositionLipids & Proteins (also carbohydrates)

Lipid Bilayer ⭐⭐

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COMPONENT POSITION NATURE
Polar heads Outer sides (facing aqueous environment) Hydrophilic
Non-polar tails Inner side (sandwiched between) Hydrophobic
Major lipids Phospholipids (Phosphoglycerides)
Also contains Cholesterol

Proteins ⭐⭐

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TYPE LOCATION DETAIL
Integral (Intrinsic) Partially or totally buried in the membrane Deeply embedded
Peripheral (Extrinsic) Lie on the surface of membrane Easily extracted

Fluidity ⭐⭐

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FEATURE DETAIL
BasisQuasi-fluid nature of lipids
ConsequenceEnables lateral movement of proteins within the lipid bilayer

Human RBC Membrane Composition ⭐

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COMPONENT PERCENTAGE
Protein~52%
Lipid~40%
⚡ EXAM TRAP

NEET 2012, 2026 (reinforced): Membrane of human RBC has approximately 52% protein and major phospholipids are arranged in a bilayer with hydrophobic tails facing inward.

⚡ EXAM TRAP

NEET 2026: Glycocalyx is present on the outer surface of plasma membrane in prokaryotes.

Functions of Fluid Nature of Membrane ⭐

• Cell growth  • Secretion  • Endocytosis  • Cell division  • Formation of intercellular junctions

Transport Across Membrane ⭐⭐

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TYPE DIRECTION ENERGY REQUIRED EXAMPLES
Passive transport High → Low conc. (along gradient) No Simple diffusion, Osmosis, Facilitated diffusion
Active transport Low → High conc. (against gradient) Yes (ATP) Na⁺/K⁺ Pump
TRANSPORT MODE DETAIL
Simple diffusionNeutral solutes move along conc. gradient
OsmosisMovement of water by diffusion (high to low concentration)
Facilitated diffusion Polar molecules cannot pass through nonpolar lipid bilayer → require carrier proteins
Active transport Against concentration gradient; ATP-dependent; e.g., Na⁺/K⁺ Pump
⚡ EXAM TRAP

NEET 2021: Active transport — Na⁺/K⁺ Pump; requires ATP; against concentration gradient.

B    CELL WALL (Eukaryotic — Plants)

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FEATURE DETAIL
NatureNon-living, rigid structure
PositionOuter covering for plasma membrane (in fungi & plants)

Composition in Different Groups ⭐

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GROUP CELL WALL COMPOSITION
Algae Cellulose, Galactans, Mannans, Minerals (CaCO₃)
Other plants Cellulose, Hemicellulose, Pectins, Proteins
Bacteria Peptidoglycan

Primary vs Secondary Cell Wall ⭐

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FEATURE PRIMARY CELL WALL SECONDARY CELL WALL
Found inYoung plant cellsMature cells
GrowthCapable of growth Growth capacity diminished
PositionOuterFormed on inner side (towards membrane)

Middle Lamella ⭐⭐

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FEATURE DETAIL
CompositionMainly Calcium pectate (Ca²⁺ pectates)
FunctionHolds / glues neighbouring cells together

Plasmodesmata ⭐: Traverse cell wall & middle lamella; connect cytoplasm of neighbouring cells.
Functions of Cell Wall ⭐: Gives shape to cell; protects from mechanical damage & infection; helps in cell-to-cell interaction; provides barrier to undesirable macromolecules.

C    ENDOMEMBRANE SYSTEM

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FEATURE DETAIL
DefinitionDifferent membranous organelles with coordinated functions
IncludesER, Golgi complex, Lysosomes, Vacuoles
Does NOT include Mitochondria, Chloroplast, Peroxisomes ⭐⭐ (functions NOT coordinated with endomembrane system)
⚡ EXAM TRAP

NEET 2021, 2023, RE-NEET 2026 (reinforced): Endomembrane system includes endoplasmic reticulum, Golgi complex, lysosomes and vacuole — mitochondria, chloroplast and peroxisomes are NOT part of it.

C1. Endoplasmic Reticulum (ER) ⭐⭐⭐

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FEATURE DETAIL
StructureNetwork of tiny tubular structures scattered in cytoplasm
FunctionDivides intracellular space into two compartments: Luminal (inside ER) & Extra-luminal (cytoplasm)

Two Types of ER ⭐⭐⭐

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TYPE FEATURE MAJOR FUNCTION
RER (Rough ER) Ribosomes attached on outer surface Protein synthesis & secretion ; Glycoprotein formation
SER (Smooth ER) No ribosomes (smooth appearance) Lipid synthesis (including steroidal hormones in animal cells)

RER is extensive & continuous with outer membrane of nucleus.
• RER frequent in cells actively involved in protein synthesis & secretion.

⚡ EXAM TRAP

NEET 2013, 2015, 2018, RE-NEET 2026 (reinforced): Smooth ER is major site for synthesis of lipids (steroidal hormones in animal cells). Rough ER bears ribosomes on its surface (protein synthesis). Lipid synthesis does NOT occur in RER!

C2. Golgi Apparatus ⭐⭐⭐

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FEATURE DETAIL
Discovered byCamillo Golgi (1898)
StructureMany flat, disc-shaped sacs called Cisternae
Cisternae diameter0.5–1.0 µm
ArrangementStacked parallel to each other; concentrically arranged near nucleus

Cis & Trans Faces ⭐⭐⭐

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FACE SHAPE ALTERNATE NAME FACES TOWARDS
Cis face Convex Forming face Towards ER / Nucleus
Trans face Concave Maturing face Towards plasma membrane
KEY FACT DETAIL
Cis & Trans facesEntirely different but interconnected
Close association withEndoplasmic Reticulum
Materials from ER Fuse with cis face → move towards trans face → released from trans face
Proteins modified In cisternae of Golgi → released from trans face (NOT cis face)

Functions ⭐⭐

Packaging of materials (for intracellular delivery or secretion outside cell)
• Modification of proteins synthesised by ER ribosomes
⭐⭐ Major site of formation of Glycoproteins & Glycolipids (Glycosylation)

⚡ EXAM TRAP

NEET 2014, 2015, 2018, 2019, 2020, 2021, 2023, 2024, 2025 (reinforced): Vesicles from ER fuse with cis face of Golgi, are modified in cisternae, and released from trans face for packaging and delivery; Golgi = major site for glycoprotein & glycolipid formation.

C3. Lysosomes ⭐⭐⭐

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FEATURE DETAIL
StructureMembrane-bound vesicular structures
Formed byProcess of packaging in the Golgi apparatus ⭐⭐ (NOT ER)
EnzymesRich in hydrolytic enzymes (hydrolases) — lipases, proteases, carbohydrases
Optimal pHAcidic pH (enzymes inactive at alkaline pH)
Can Digest ⭐Carbohydrates, Proteins, Lipids, Nucleic acids
⚡ EXAM TRAP

NEET 2016, 2019, 2022: Lysosomes — formed by packaging in Golgi (NOT ER); contain hydrolytic enzymes active at acidic pH (inactive at alkaline pH!).

C4. Vacuoles ⭐⭐

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FEATURE DETAIL
StructureMembrane-bound space in cytoplasm
MembraneBound by a single membrane called Tonoplast ⭐⭐
In plant cellsCan occupy up to 90% of cell volume
Contents ⭐Water, Cell sap, Excretory products, Other materials not useful for cell

Tonoplast Function ⭐⭐

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FEATURE DETAIL
FunctionFacilitates transport of ions & materials against concentration gradient into the vacuole
ResultConcentration significantly higher in vacuole than in cytoplasm

Special Vacuoles ⭐

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ORGANISM VACUOLE TYPE FUNCTION
AmoebaContractile vacuoleOsmoregulation & excretion
ProtistsFood vacuolesFormed by engulfing food particles

D    MITOCHONDRIA

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FEATURE DETAIL
VisibilityNot easily visible without specific staining
NumberVariable — depends on physiological activity of cell
ShapeSausage-shaped or cylindrical
Diameter & LengthDiameter 0.2–1.0 µm (average 0.5 µm); Length 1.0–4.1 µm
MembraneDouble membrane-bound ⭐⭐
DivisionDivide by fission

Structure ⭐⭐⭐

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COMPONENT DETAIL
Outer membrane Continuous limiting boundary; more permeable (permeable to monomers)
Inner membrane Forms infoldings called Cristae ⭐⭐; relatively less permeable
Two aqueous compartments Outer compartment (perimitochondrial space) & Inner compartment (matrix)
Cristae Infoldings of inner membrane towards matrix ; increase surface area
MatrixDense, homogeneous substance filling inner compartment

Matrix Contents ⭐⭐

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CONTENT DETAIL
DNASingle, circular, double-stranded DNA molecule
RNAA few RNA molecules
Ribosomes70S type (required for protein synthesis within mitochondria)
EnzymesComponents for protein synthesis

Function ⭐⭐⭐

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FEATURE DETAIL
Main functionSite of aerobic respiration
ProductCellular energy in the form of ATP
Called'Power Houses' of the cell ⭐⭐
⚡ EXAM TRAP

NEET 2015, 2016, 2017, 2019, 2021, 2024, 2026, RE-NEET 2026 (reinforced): Mitochondrial inner membrane encloses matrix; cristae increase surface area; both mitochondria and plastids have circular DNA and are double membrane bound. Mitochondria = 'Power Houses' of cell!

E    PLASTIDS

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FEATURE DETAIL
Found inAll plant cells & Euglenoids
SizeLarge — easily observed under microscope

Three Types (Based on Pigments) ⭐⭐

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TYPE PIGMENTS COLOUR GIVEN FUNCTION / DETAIL
Chloroplast Chlorophyll & Carotenoid Green Trapping light energy for photosynthesis
Chromoplast Fat-soluble carotenoid pigments (Carotene, Xanthophyll, etc.) Yellow, Orange, Red Give colour to plant parts
Leucoplast No pigments (Colourless) Storage of nutrients
⚡ EXAM TRAP

NEET 2013, 2024, RE-NEET 2026 (reinforced): Chromoplast is a plastid that stores xanthophyll/carotene (fat-soluble carotenoids giving yellow, orange, red colour).

Leucoplast — Three Subtypes ⭐⭐

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SUBTYPE STORES EXAMPLE
AmyloplastCarbohydrates (Starch) Potato
ElaioplastOils & Fats
AleuroplastProteins

Chloroplast — Detailed Structure ⭐⭐⭐

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FEATURE DETAIL
ShapesLens-shaped, Oval, Spherical, Discoid, Ribbon-like
Length & WidthLength 5–10 µm; Width 2–4 µm
Number 1 per cell in Chlamydomonas ; 20–40 per cell in mesophyll cells
MembraneDouble membrane-bound ; Inner membrane relatively less permeable

Internal Structure ⭐⭐

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COMPONENT DETAIL
Stroma Space limited by inner membrane; contains enzymes for carbohydrate & protein synthesis ; contains circular dsDNA , 70S ribosomes
Thylakoids Flattened membranous sacs in stroma ; arranged in stacks like piles of coins = Grana
Grana (sing.: Granum) Stacks of thylakoids ; site of light reactions
Stroma lamellae Connect thylakoids of different grana
Thylakoid lumen Space enclosed by thylakoid membrane
Chlorophyll Present in thylakoids (NOT in stroma)
DNA Small, double-stranded, circular
Ribosomes 70S (smaller than cytoplasmic 80S)
Stroma Site of dark reactions
⚡ EXAM TRAP

NEET 2014, 2015, 2021, 2024: Chloroplast structure — double membrane, stroma contains circular dsDNA and 70S ribosomes; thylakoids stacked into grana.

F    RIBOSOMES

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FEATURE DETAIL
Discovered byGeorge Palade (1953)
CompositionRNA + Proteins
MembraneNOT surrounded by any membrane
FunctionProtein synthesis ('Protein factory') ⭐⭐

Two Types ⭐⭐

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TYPE FOUND IN SUBUNITS
80S Eukaryotes (cytoplasm) 60S + 40S
70S Prokaryotes, also in Mitochondria & Chloroplasts 50S + 30S

'S' unit = Svedberg unit — indirectly measures density & size (sedimentation coefficient).
Location of Ribosomes in Eukaryotic Cells ⭐: Free in cytoplasm, attached to RER, within Chloroplasts, within Mitochondria.

⚡ EXAM TRAP

NEET 2019, 2025, 2026, RE-NEET 2024 (reinforced): Eukaryotic ribosomes are 80S with 60S and 40S subunits, prokaryotic ribosomes are 70S with 50S and 30S subunits — each ribosome has two subunits. Ribosomes are non-membrane bound organelles found in both prokaryotic and eukaryotic cells!

G    CYTOSKELETON

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FEATURE DETAIL
Composition Elaborate network of filamentous proteinaceous structures

Three Components ⭐

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COMPONENT DETAIL
Microtubules Constitute spindle fibres, centrioles, cilia, flagella
Microfilaments Solid linear elements; diameter ~6 nm; made of actin monomer
Intermediate filaments

Functions ⭐⭐: Mechanical support, Motility, Maintenance of cell shape.

⚡ EXAM TRAP

NEET 2014, 2016, 2023, 2026 (reinforced): Network of microtubules, microfilaments and intermediate filaments in cytoplasm is called cytoskeleton; functions = mechanical support, motility, shape maintenance.

H    CILIA & FLAGELLA

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FEATURE CILIA FLAGELLA
NatureHair-like outgrowths of cell membraneHair-like outgrowths of cell membrane
SizeSmall Longer than cilia
FunctionWork like oars → move cell or surrounding fluidResponsible for cell movement
Emerge fromBasal body Basal body
CoveringCovered with plasma membraneCovered with plasma membrane

Axoneme (Core Structure) ⭐⭐⭐

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FEATURE DETAIL
Core calledAxoneme
Microtubule arrangement9 + 2 array ⭐⭐
9 peripheralDoublets (radially arranged)
2 centralSinglets
Central tubulesConnected by bridges; enclosed by central sheath
Radial spokes9 in number; connect central sheath to one tubule of each peripheral doublet
Peripheral doubletsConnected by linkers
⚡ EXAM TRAP

NEET 2024: Axoneme = core of cilia/flagella; 9+2 arrangement of microtubules.

I    CENTROSOME & CENTRIOLES

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FEATURE DETAIL
CentrosomeOrganelle containing two cylindrical structures called centrioles
Centriole orientationTwo centrioles lie perpendicular to each other
Surrounding materialAmorphous pericentriolar material
Centriole organisationLike a Cartwheel ⭐⭐

Centriole Structure ⭐⭐

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FEATURE DETAIL
Peripheral fibrils9 evenly spaced peripheral fibrils of tubulin protein
Each fibrilA triplet (contrast: cilia/flagella = doublets)
Adjacent tripletsLinked to each other
Central partHub (proteinaceous; in proximal region)
Hub ↔ peripheral tripletsConnected by radial spokes (protein)
Microtubule arrangement9 + 0 ⭐⭐ (NO central tubules — contrast with cilia/flagella 9+2)
FEATURE DETAIL
Functions ⭐⭐Form basal body of cilia & flagella; Form spindle fibres during cell division in animal cells ⭐⭐
In plant cellsAbsent in almost all plant cells
Non-membrane-boundYes
⚡ EXAM TRAP

NEET 2014, 2020, 2024: Centriole = Cartwheel organisation; 9+0 arrangement; forms basal body of cilia/flagella & spindle fibres.

Cilia/Flagella vs Centriole — Microtubule Comparison ⭐⭐

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FEATURE CILIA / FLAGELLA CENTRIOLE
Arrangement9 + 29 + 0
Peripheral typeDoubletsTriplets
Central microtubules2 singlets (present)Absent (hub instead)

J    NUCLEUS

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FEATURE DETAIL
First described byRobert Brown (1831)
Chromatin named byFlemming

Nuclear Envelope ⭐⭐

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FEATURE DETAIL
StructureTwo parallel membranes (double membrane-bound)
Perinuclear spaceSpace between the two membranes (10–50 nm) ; forms barrier between nucleus & cytoplasm
Outer membraneContinuous with ER ; bears ribosomes on it
Nuclear poresFormed by fusion of both membranes
Pore functionPassage for movement of RNA & Protein molecules (both directions)
⚡ EXAM TRAP

NEET 2015, 2021: Nuclear envelope outer membrane continuous with ER and bears ribosomes; Nuclear pores allow passage of RNA & protein in both directions.

Number of Nuclei ⭐

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CONDITION EXAMPLE
NormallyOne nucleus per cell
More than oneParamecium, Liquid endosperm of coconut
No nucleus (at maturity) RBCs of many mammals ; Sieve tube cells of vascular plants

Interphase Nucleus ⭐: = Nucleus of a cell when it is NOT dividing. Contains: Chromatin, Nuclear matrix (Nucleoplasm), Nucleoli (one or more).

Nucleolus ⭐⭐

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FEATURE DETAIL
ShapeSpherical
MembraneNon-membrane-bound
ContentContinuous with rest of nucleoplasm
FunctionSite of active ribosomal RNA (rRNA) synthesis ⭐⭐
Size & numberLarger & more numerous in cells actively carrying out protein synthesis
⚡ EXAM TRAP

NEET 2018, 2020, 2024, 2026 (reinforced): Nucleolus — non-membrane-bound; site for active ribosomal RNA (rRNA) synthesis; larger in cells with active protein synthesis.

Chromatin ⭐⭐⭐

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FEATURE DETAIL
StructureHighly extended & elaborate nucleoprotein fibres
During cell divisionCondense → form chromosomes
ContainsDNA + Basic proteins (Histones) + Some non-histone proteins + RNA
Human cell~2 metres of DNA thread distributed among 46 chromosomes (23 pairs)
⚡ EXAM TRAP

NEET 2015, RE-NEET 2024: Chromatin = DNA + Histones + Non-histone proteins + RNA.

Chromosomes ⭐⭐⭐

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FEATURE DETAIL
Primary constriction= Centromere
KinetochoresDisc-shaped structures on sides of centromere
Centromere functionHolds two chromatids of a chromosome together
Secondary constrictionNon-staining; at constant location
SatelliteFragment / part of chromosome after secondary constriction

Types Based on Centromere Position ⭐⭐⭐

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TYPE CENTROMERE POSITION ARMS
MetacentricMiddle Two equal arms
Sub-metacentricSlightly away from middle One shorter arm + one longer arm
AcrocentricClose to one end One extremely short arm + one very long arm
TelocentricTerminal (at the end) One arm only
⚡ EXAM TRAP

NEET 2021, 2022, 2024, RE-NEET 2024: Chromosome classification based on centromere position — know all four types!

K    MICROBODIES

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FEATURE DETAIL
StructureMembrane-bound minute vesicles
ContentsVarious enzymes
Present inBoth plant & animal cells
⚡ EXAM TRAP

NEET 2021: Microbodies — membrane-bound; contain enzymes; present in both plant & animal cells.

V. RAPID REVISION — KEY COMPARISON TABLES

TABLE 1: Scientists & Discoveries ⭐⭐⭐

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SCIENTIST DISCOVERY YEAR
Robert HookeFirst cell (dead cork)1665
LeeuwenhoekFirst live cell
Robert BrownNucleus1831
FlemmingChromatin (named)
Schleiden + SchwannCell Theory1838–39
VirchowOmnis cellula-e cellula1855
Singer & NicolsonFluid Mosaic Model1972
Camillo GolgiGolgi bodies1898
George PaladeRibosomes1953
G.N. RamachandranTriple helix of collagen; Ramachandran plot1954

TABLE 2: Prokaryotic vs Eukaryotic Cell ⭐⭐⭐

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FEATURE PROKARYOTIC EUKARYOTIC
Nuclear membraneAbsentPresent
Membrane-bound organellesAbsentPresent
Ribosomes70S (50S + 30S)80S (60S + 40S)
DNACircular, nakedLinear, with histones
MesosomesPresentAbsent
PlasmidsPresentAbsent
Cell wallPresent (except Mycoplasma)Plants/Fungi (present); Animals (absent)

TABLE 3: Plant Cell vs Animal Cell ⭐⭐⭐

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FEATURE PLANT CELL ANIMAL CELL
Cell wallPresent (cellulose)Absent
PlastidsPresentAbsent
Large central vacuolePresentAbsent / Small
CentriolesAbsentPresent
PlasmodesmataPresentAbsent
LysosomesFew / less prominentProminent

TABLE 4: Endomembrane System Components ⭐⭐⭐

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ORGANELLE MEMBRANE TYPE KEY FUNCTION
ER (RER)SingleProtein synthesis & secretion
ER (SER)SingleLipid synthesis (steroidal hormones)
Golgi apparatusSingle (Cisternae)Packaging, modification; Glycoprotein / Glycolipid formation
LysosomesSingleDigestion (hydrolytic enzymes at acidic pH)
VacuolesSingle (Tonoplast)Storage; osmoregulation (Amoeba)

TABLE 5: Double Membrane-Bound Organelles — Comparison ⭐⭐⭐

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FEATURE MITOCHONDRIA CHLOROPLAST
Found inAll eukaryotesPlants & Euglenoids
MembraneDoubleDouble
Inner membrane formsCristae Limits Stroma
Internal structuresMatrix (inside)Thylakoids, Grana (stacked thylakoids)
DNACircular, dsDNACircular, dsDNA
Ribosomes70S70S
FunctionAerobic respiration (ATP)Photosynthesis
Called'Power House'
DivisionFission
Semi-autonomousYesYes

TABLE 6: Cilia/Flagella vs Centriole ⭐⭐⭐

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FEATURE CILIA / FLAGELLA CENTRIOLE
Microtubule arrangement9 + 29 + 0
Peripheral typeDoubletsTriplets
Central tubules2 singlets (present)Absent (Hub present)
FunctionMovementBasal body + Spindle fibres

TABLE 7: Ribosomes ⭐⭐⭐

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FEATURE 70S 80S
Found inProkaryotes, Mitochondria, ChloroplastEukaryotic cytoplasm
Subunits50S + 30S60S + 40S
FunctionProtein synthesisProtein synthesis

TABLE 8: Cell Sizes ⭐⭐⭐

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CELL / ORGANISM SIZE
Mycoplasma0.3 µm (smallest cell)
PPLO~0.1 µm
Virus0.02–0.2 µm
Typical bacterium1–2 µm
Human RBC~7 µm
Typical eukaryotic cell10–20 µm
Largest cellOstrich egg
Longest cellNerve cell

TABLE 9: Chromosome Types ⭐⭐⭐

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TYPE CENTROMERE POSITION ARMS
MetacentricMiddleEqual
Sub-metacentricSlightly off-centreOne short + one long
AcrocentricNear one endOne very short + one very long
TelocentricTerminalEssentially one arm

VI. COMMON EXAM TRAPS — QUICK REFERENCE

NEET 2025, 2026 & RE-NEET 2026 REINFORCED TRAPS

RE-NEET 2026: Cell theory was formulated by Schleiden and Schwann.
NEET 2025: Reductionist Biology is the physico-chemical approach to study living organisms.
RE-NEET 2026: Prokaryotic ribosomes are 70S made of 50S and 30S subunits.
RE-NEET 2026: Prokaryotes can have plasmids and contain mesosomes; peroxisomes are absent in prokaryotes.
NEET 2026: Ribosomes are non-membrane bound organelles found in both prokaryotic and eukaryotic cells.
NEET 2025: Mesosome is a specialised membranous structure in prokaryotic cells which helps in cell wall formation and DNA replication.
NEET 2026: Extensions of plasma membrane into the cell forming mesosomes occur in prokaryotes — not in eukaryotic cell membranes.
NEET 2026: Membrane of human RBC has approximately 52% protein and major phospholipids are arranged in a bilayer with hydrophobic tails facing inward.
NEET 2026: Glycocalyx is present on outer surface of plasma membrane in prokaryotes.
RE-NEET 2026: Endomembrane system includes endoplasmic reticulum, Golgi complex, lysosomes and vacuole — mitochondria, chloroplast and peroxisomes are not part of it.
RE-NEET 2026: Smooth endoplasmic reticulum is major site for synthesis of lipids.
NEET 2026: Rough endoplasmic reticulum bears ribosomes on its surface.
NEET 2025: Vesicles from endoplasmic reticulum fuse with cis face of Golgi and are modified and released from trans face for packaging and delivery.
RE-NEET 2026: Mitochondrial inner membrane encloses matrix.
NEET 2026: Both mitochondria and plastids have circular DNA and mitochondrion is double membrane bound.
RE-NEET 2026: Chromoplast is a plastid that stores xanthophyll.
NEET 2025: Eukaryotic ribosomes are 80S with 60S and 40S subunits, prokaryotic ribosomes are 70S with 50S and 30S subunits — each ribosome has two subunits.
NEET 2026: Network of microtubules, microfilaments and intermediate filaments in cytoplasm is called cytoskeleton.
NEET 2026: Nucleolus is site for active ribosomal RNA synthesis.

CONSOLIDATED PYQ Q&A TABLE

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TRAP / QUESTION CORRECT ANSWER
Robert Hooke or Robert Brown — who discovered cell?Robert Hooke discovered cell; Robert Brown discovered nucleus
Schwann — British or German?German Zoologist (NCERT says German)
Omnis cellula-e cellula — who said?Rudolf Virchow (1855) ⭐⭐
Fluid Mosaic Model — who proposed?Singer & Nicolson (1972)
Smallest cell?Mycoplasma (0.3 µm)
Largest cell?Ostrich egg
Longest cell?Nerve cell
Ribosomes — membrane-bound?No (non-membrane-bound; present in both pro & eukaryotes)
Centrioles — present in plant cells?No (absent in almost all plant cells)
Mitochondria, Chloroplast — part of endomembrane?No — their functions are NOT coordinated with it ⭐⭐
Endomembrane system includes?ER, Golgi, Lysosomes, Vacuoles
RER function?Protein synthesis & secretion
SER function?Lipid synthesis (steroidal hormones in animal cells)
Lipid synthesis in RER?No — occurs in SER
Golgi — cis or trans face releases proteins?Trans face (maturing face) releases; Cis face (forming face) receives from ER
Golgi — cis face shape?Convex
Golgi — trans face shape?Concave
Golgi — major site for?Glycoprotein & Glycolipid formation ⭐⭐
Lysosomes formed by?Packaging in Golgi apparatus (NOT ER)
Lysosomal enzymes active at?Acidic pH (inactive at alkaline pH)
Tonoplast = ?Vacuolar membrane ⭐⭐
Vacuole occupies what % in plant cells?Up to 90% of cell volume
Contractile vacuole — in?Amoeba (osmoregulation & excretion)
Food vacuoles — in?Protists
Mitochondria called?'Power Houses' of the cell ⭐⭐
Cristae = ?Infoldings of inner membrane of mitochondria
Mitochondria DNA — circular or linear?Circular, double-stranded
Mitochondria ribosomes — 70S or 80S?70S
Mitochondria divide by?Fission
Chloroplast inner membrane permeable?Relatively less permeable than outer
Stroma contains?Enzymes + circular dsDNA + 70S ribosomes
Thylakoids = ?Flattened membranous sacs in stroma
Grana = ?Stacked thylakoids (piles of coins)
Chlorophyll is in stroma or thylakoids?Thylakoids
Light reactions occur in?Grana (thylakoids)
Dark reactions occur in?Stroma
Chloroplast ribosomes?70S (smaller than cytoplasmic 80S)
80S ribosomes subunits?60S + 40S
70S ribosomes subunits?50S + 30S
'S' stands for?Svedberg unit (sedimentation coefficient)
Polysomes / Polyribosomes = ?Several ribosomes on a single mRNA
George Palade discovered?Ribosomes (1953)
Cytoskeleton components?Microtubules + Microfilaments + Intermediate filaments
Microfilaments made of?Actin protein; diameter ~6 nm
Cilia/Flagella axoneme arrangement?9 + 2 (doublets + singlets) ⭐⭐
Centriole arrangement?9 + 0 (triplets; no central tubules) ⭐⭐
Centriole looks like?Cartwheel
Hub is in?Proximal region of centriole
Cilia emerge from?Basal body (centriole-like structure)
Nuclear pores allow passage of?RNA & Protein (both directions)
Outer nuclear membrane continuous with?ER (and bears ribosomes)
Perinuclear space?Space between two nuclear membranes (10–50 nm)
Cells lacking nucleus (mature)?Mammalian RBCs & Sieve tube cells
Multi-nucleate examples?Paramecium, Liquid endosperm of coconut
Nucleolus — membrane-bound?No (non-membrane-bound)
Nucleolus function?rRNA synthesis ⭐⭐
Chromatin = ?DNA + Histones + Non-histone proteins + RNA
Centromere = ?Primary constriction of chromosome
Kinetochore = ?Disc-shaped structure on sides of centromere
Satellite = ?Fragment after secondary constriction
Metacentric centromere position?Middle (equal arms)
Telocentric centromere position?Terminal (at end)
Acrocentric centromere position?Close to one end
Microbodies — found in?Both plant & animal cells
Mycoplasma cell wall?Absent (wall-less)
Bacterial cell wall composition?Peptidoglycan
Mesosomes — what are they?Extensions of plasma membrane (vesicles, tubules, lamellae)
Mesosomes function?Cell wall formation, DNA replication, respiration, secretion, increase surface area
Pili & Fimbriae — motility?No — help in attachment only
Inclusion bodies — membrane-bound?No — lie free in cytoplasm
Gas vacuoles found in?Blue-green, purple & green photosynthetic bacteria
Chromatophores found in?Cyanobacteria — membranous extensions with pigments
Cell wall of algae?Cellulose, Galactans, Mannans, CaCO₃
Middle lamella composition?Calcium pectate
Plasmodesmata function?Connect cytoplasm of neighbouring cells
Facilitated diffusion needed for?Polar molecules (can't cross nonpolar lipid bilayer)
Na⁺/K⁺ Pump = ?Active transport (against gradient; requires ATP)
RBC membrane composition?~52% protein + ~40% lipid
Quasi-fluid nature of lipids enables?Lateral movement of proteins in membrane
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